FastQCFastQC Report
Sun 14 Apr 2019
SRR6576251_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576251_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283632
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6180.21788796750719241No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.15054718790545496No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3680.12974558582952558No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3070.10823884470017486No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2900.10224516274609353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAGC957.2759576E-1249.4603238
GTATCAA7500.047.6305961
GTACATG17550.046.066481
CATGGGT6200.044.7137264
TACATGG18400.043.6829572
ACATGGG18150.043.23353
GGGTTAG1251.5097612E-1037.5898447
ATGGGTA3150.037.291515
GTTAGCA1302.2919266E-1036.144089
CATGGGG7600.035.8587344
ATCAACG10050.035.0651553
TCAACGC10150.034.7196854
CAACGCA10450.033.7229465
AACGCAG10950.032.6121946
GGTACCT3150.031.3248718
TATACTG902.5918729E-531.3248715
CCTATCC753.2745537E-431.3248713
TATCAAC11700.030.9341772
TGGGTAC3200.030.835426
ATGGGTT2051.8189894E-1229.796835
Produced by FastQC (version 0.11.2)