FastQCFastQC Report
Sun 14 Apr 2019
SRR6576248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237287
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8430.35526598591578973No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4530.19090805648855605No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4450.1875366117823564No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3880.16351506825068376No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3380.14244353883693586No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3150.13275063530661182No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3100.130643482365237No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3090.13022205177696208No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3080.1298006211886871No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2990.12600774589421251No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2840.1196862870700881No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2830.11926485648181316No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2810.11842199530526325No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2810.11842199530526325No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2710.11420768942251365No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2660.11210053648113888No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2630.110836244716314No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2500.10535764706873954No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2430.10240763295081483No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2400.10114334118598997No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2380.10030048000944006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT250.00522397356.4189721
TTTAGGC450.001012752141.774223
GTATCAA8100.037.728741
ACATGGG14550.035.8526153
GTACATG15050.035.6133041
TACATGG15350.034.9172822
TTAGGCA550.00271381834.1789054
GTAGTGT702.180891E-433.568574
AACGCAG9350.032.168386
ATCAACG10050.030.3954183
ACGCAGA9950.030.2285797
TCAACGC10000.030.0774354
CATGGGG7300.029.6139164
CAACGCA10300.029.2013955
TAATGGG650.00614702128.9206124
CGCAGAG10450.028.7822368
CATGGGT3950.028.554534
TAACGCC856.803431E-427.6447034
AACGCCG856.803431E-427.6447035
GAGTGCG700.00882581626.8548557
Produced by FastQC (version 0.11.2)