FastQCFastQC Report
Sun 14 Apr 2019
SRR6576247_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576247_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593658
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10650.17939621802451916No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7710.1298727550205674No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7200.12128195021375943No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC6410.10797462512086083No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6040.10174208045709819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13200.049.5158961
CAACGCA17450.035.5518955
TCAACGC17550.035.349324
ATCAACG17650.035.1490363
AACGCAG17750.034.4214556
TACATGG30200.033.6176722
GTACATG30450.033.510141
ACATGGG31550.032.474423
CATGGGG18450.031.5870864
GTATTAA1201.6431659E-731.3481941
TATCAAC20950.030.0636432
ACGCAGA22300.027.3982437
ATAACGC2600.027.1145343
AATAACG2650.026.605182
ATGGGGG7450.026.4958135
CGCAGAG23250.026.4808928
TATAGGA1501.1604134E-625.0679932
TAACGCC3000.025.0658824
AACGCCG2850.024.7360675
TATAATA1751.6425474E-724.1727072
Produced by FastQC (version 0.11.2)