FastQCFastQC Report
Sun 14 Apr 2019
SRR6576246_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576246_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390941
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6170.15782432643288885No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.137872466689347No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4200.1074330909267639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12300.039.0045931
ATACTAC500.001701927537.594276
TACATGG25700.034.193232
ACATGGG25750.033.7618453
GGTATAC702.1728661E-433.596391
GTACATG26650.033.1803781
GTACTAT600.004142552631.3566341
AACGCAG16500.029.050126
ATCAACG16350.029.0292153
TCAACGC16400.028.9407124
CATGGGT6350.028.1216964
CAACGCA16950.028.0016335
GGGTACC2900.027.5475257
GGTACCT2800.026.8736748
CATGGGA15450.026.7661484
TATCAAC17750.026.7395862
CATGGGG8650.025.5336824
TATAGTA1851.0009899E-825.4015352
ACGCAGA19700.024.5698597
GTACAAC1151.3680167E-424.5399741
Produced by FastQC (version 0.11.2)