FastQCFastQC Report
Sun 14 Apr 2019
SRR6576246_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576246_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390941
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7730.19772804591997256No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5000.12789653681757604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG25400.037.561662
GTACATG25700.037.3060761
ACATGGG25200.037.2907263
GTATCAA12550.036.3254051
CATGGGT4700.035.9895174
ATGGGAT4100.033.2342225
CATGGGG9800.033.08224
AACGCAG14650.030.468946
ATCAACG15300.029.481613
CATGGGA13950.029.3032914
TCAACGC15500.028.7980634
ATGGGGG4250.028.7445685
CAACGCA15550.028.7054655
ACGCAGA16500.027.0527257
GGTACCT2800.026.8493218
ACCTAGC700.00884156826.8493218
TGCGAAA350.00884688126.84932112-13
TATCAAC17750.026.2131582
GGGTACC2700.026.103517
CGCAGAG17100.026.1035088
Produced by FastQC (version 0.11.2)