FastQCFastQC Report
Sun 14 Apr 2019
SRR6576245_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576245_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430214
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5690.13225975909663565No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5000.11622122943465345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11450.039.53341
TACATGG24850.036.12352
GTACATG25200.035.7380871
GAATCCG550.00271558934.1806349
CCGTGCA550.00271558934.1806349
ACATGGG26300.033.5957953
CATGGGG8400.033.5702674
ATGGGCG851.7526572E-533.175325
ATGGGTA3400.029.0284065
ATCAACG15250.028.9694883
TCAACGC15300.028.8748174
AACGCAG15350.028.4745866
CAACGCA15950.027.9927625
CATGGGT6300.026.8562134
CTACTCT1606.897244E-826.4365844
TATCAAC17400.025.3899272
CGCGGTA1301.0860576E-525.30681894
ATACCGT950.001303522524.7359876
ACGCAGA18450.023.6902377
ATGGGAG6800.023.4991865
Produced by FastQC (version 0.11.2)