FastQCFastQC Report
Sun 14 Apr 2019
SRR6576244_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576244_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147659
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5750.38941073690056144No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.23432367820451172No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3290.22281066511353861No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3210.2173927766001395No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2840.1923350422256686No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2830.1916578061614937No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2510.16998625210789725No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2430.16456836359449814No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2330.15779600295274923No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2330.15779600295274923No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2240.15170087837517524No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2210.14966917018265055No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2210.14966917018265055No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2200.14899193411847567No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2060.13951062922002724No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT1990.134769976770803No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC1840.12461143580817967No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1820.12325696367982987No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT1820.12325696367982987No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG1740.11783907516643077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.11445289484555632No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1670.11309842271720655No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG1610.10903500633215721No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT1590.10768053420380742No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG1580.10700329813963254No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC1560.10564882601128275No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG1530.10361711781875808No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG1480.10023093749788364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAG255.547406E-575.1888963
ATGGGAT1650.039.87295
GTATCAA6500.039.133151
GTACATG13050.038.622261
CAGATGT500.0016984637.5944484
GGGTTAG500.0016984637.5944487
TACATGG13200.037.3808442
ACATGGG13050.036.3701063
ACATATA651.4087567E-436.1485062
TGGGAGT801.1530003E-535.2447936
CATGGGT3300.034.176774
ATGGGTA2350.033.994985
CATGGGA5450.032.76584
CATGGGC1606.002665E-1132.3077284
GGTACCT1900.032.1749348
AACGCAG7900.031.5269896
TCTAACT300.004159027231.32870736-37
GGGTACC1950.031.3287037
GTACTTT7200.030.7489151
ATGGGGC1701.200533E-1030.407275
Produced by FastQC (version 0.11.2)