FastQCFastQC Report
Sun 14 Apr 2019
SRR6576244_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576244_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147659
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5610.379929432002113No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3390.22958302575528752No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3250.22010172085683907No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2820.19098057009731884No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2790.18894886190479415No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.18285373732722016No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2620.17743584881382105No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2490.16863177997954748No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2280.15440982263187478No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2240.15170087837517524No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1970.13341550464245322No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1930.13070656038575368No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC1910.12935208825740388No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC1870.12664314400070434No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG1860.12596590793652943No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1820.12325696367982987No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG1760.11919354729478053No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1740.11783907516643077No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT1710.1158073669739061No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT1680.11377565878138143No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG1680.11377565878138143No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1650.11174395058885676No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA1590.10768053420380742No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT1580.10700329813963254No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG1570.10632606207545764No Hit
CTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTA1550.10497158994710787No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1550.10497158994710787No Hit
GAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAA1500.10158540962623341No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC1480.10023093749788364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA201.8349998E-593.9749454
CGCTAAA201.8349998E-593.9749455
GACTTAT200.002161521770.481217
GGGTTAG353.6286237E-667.124967
TGCCCGC603.5652192E-1062.6924171
GGACTAG250.005232551656.3849694
CCGAACT352.9231716E-453.7363621
TGGGCAG652.7734714E-643.373056
TGGGTAT555.2689506E-542.715886
CCCGCTA450.001012411141.766643
GTACATG12600.039.1827621
CATGGGT2700.038.2860874
GTATCAA6900.038.1606061
ATGGGTA2100.038.0374765
GGTTAGC500.001699452437.5899738
TACATGG13300.037.474042
ACATGGG13350.037.3084033
ACCCTTG801.1539398E-535.2406047
AACCCTT959.396299E-734.622356
CAGTACT702.179238E-433.562484
Produced by FastQC (version 0.11.2)