FastQCFastQC Report
Sun 14 Apr 2019
SRR6576241_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576241_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481753
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11950.24805242520544762No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6810.14135874607942245No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6150.1276587794990379No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5880.1220542477161533No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5530.11478911392352512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.10191944834801238No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4830.10025884633826879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10650.049.883361
GTACATG28050.039.8906361
TACATGG28400.039.3990252
ACATGGG28550.038.685963
TCAACGC14200.035.7459644
ATCAACG14550.034.8860973
CAACGCA14850.034.497825
AACGCAG15150.033.8146976
CATGGGT7800.033.140554
ACGCAGA17100.029.4089367
TATCAAC17600.029.3837282
CATGGGG13950.029.3114074
ATGGGTA5150.029.2034635
CATGGGA11600.029.1719954
CGCAGAG18300.027.7373098
GCACCGA909.510422E-426.1107376
GGTACCT4750.025.7259468
GGGTACC5050.025.1283517
ATGGGAG6650.024.0297325
GGTATCA4900.023.9867171
Produced by FastQC (version 0.11.2)