FastQCFastQC Report
Sun 14 Apr 2019
SRR6576240_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576240_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446760
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8150.18242456800071627No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6300.14101531023368252No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4970.11124541140657175No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4570.10229205837586176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG250.005238244356.3883175
GTATCAA14700.037.7919771
CATGGGT5750.036.774994
ACATGGG29850.036.679173
TACATGG31050.036.1736872
GTACATG31450.035.7776831
ATGGGTA4000.031.7184285
AACGCAG18300.030.2997346
TCAACGC18900.029.3378354
ATCAACG18950.029.2604263
CAACGCA19050.029.1068295
GGTACCT4100.028.6718548
CATGGGA18400.028.6027684
CATGGGG10550.027.615134
ATGGGAG8000.027.6067815
GGGTACC4400.026.6990157
ACGCAGA21050.026.3413357
CGCAGAG21100.026.073728
TGGGTAC5150.025.5481016
ATATAGA1301.0716827E-525.3506831
Produced by FastQC (version 0.11.2)