FastQCFastQC Report
Sun 14 Apr 2019
SRR6576239_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576239_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495626
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9420.19006266822160256No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6210.12529609019704374No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5510.11117253735679726No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5280.10653194142357342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16150.041.748831
TATACCG500.001701506237.5981455
GTACATG34400.037.1442531
TACATGG34150.036.7448922
ACATGGG34050.036.5767563
ATGGGAG7850.036.520495
ATCAACG20350.033.02543
TCAACGC20350.033.02544
AACGCAG20450.032.8639076
CAACGCA21300.031.9937255
CATGGGG12500.031.582444
CATGGGA18250.030.387544
CTCGATA552.9690436E-629.90761664-65
CATGGGT6450.029.8744954
TATCAAC23400.029.1224922
ACGCAGA23350.028.581037
GTATAAC1005.2033294E-528.2899381
CGCAGAG23600.028.2782658
CTAGTAC1005.3192998E-528.1986063
ATGGGTA4350.028.0905675
Produced by FastQC (version 0.11.2)