FastQCFastQC Report
Sun 14 Apr 2019
SRR6576236_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576236_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489777
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7660.15639770752811996No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5110.10433319653638289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8350.050.135891
TCAACGC10250.039.891994
CAACGCA10500.038.942185
ATCAACG10800.037.8604553
AACGCAG11300.037.0170566
GTACATG19150.033.896551
TACATGG18850.032.9152572
ACATGGG19550.031.7334653
TATCAAC13250.031.5724892
ACGCAGA13150.031.4519257
GAACCGT600.00415902531.3327886
GGTATCA3950.030.961461
CGCAGAG13650.030.299848
CCCGCTA650.00615128928.9225753
CATGGGG12000.027.024534
CGCCGGT1606.9001544E-826.437047
GCAGAGT16050.025.7690229
CCTAGTA950.001302873424.738942
AGGCGTG1151.3757948E-424.5213137
GACCGCG1951.7516868E-824.1021447
Produced by FastQC (version 0.11.2)