FastQCFastQC Report
Sun 14 Apr 2019
SRR6576234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329846
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5460.16553179362490375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTG301.3701337E-462.6628277
GTATCAA7600.045.774231
ATGGGAG4450.036.9640245
ATCAACG9600.035.7373923
ATGGGTA2500.035.717815
AACGCAG10100.035.364176
GTACATG18900.034.57451
ACATGGG18850.034.1570553
TACATGG18750.034.0989152
CAACGCA10150.033.8008845
TCAACGC10250.033.471124
TATCAAC10850.032.0630572
TAGTACT1052.060453E-631.3314134
CATGGGT4950.030.3819774
ACCGTGT1102.9614057E-629.9072598
ACGCAGA11850.029.7450127
GCACCGT953.7458227E-529.6823926
ACCGTGA953.7458227E-529.6823928
CGCAGAG11900.029.6200338
GCAAACG804.7668177E-429.3821091
Produced by FastQC (version 0.11.2)