FastQCFastQC Report
Sun 14 Apr 2019
SRR6576233_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576233_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403106
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8800.21830486274081756No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5490.13619246550535094No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5460.13544824438237088No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4470.11088894732402893No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4290.10642362058614854No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4130.10245444126358823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12650.045.3729171
ACCGTCC608.788349E-539.1859558
ATCAACG15700.036.2182923
TACATGG25450.035.6378752
AACGCAG15800.035.3942036
ACATGGG25700.035.1083453
TCAACGC15850.034.9860574
CAACGCA16100.034.7346845
GTACATG26200.034.297261
GTATAGG753.252874E-431.3643361
TATCAAC18350.030.731762
CGCAGAG18650.030.5083168
ACGCAGA18600.030.0660427
CATGGGT6300.029.837454
CATGGGG9700.029.5529174
CATGGGA12450.027.1772444
ATGGGTA3850.026.8537045
GCAGAGT21300.026.7126799
GTATATA2409.094947E-1225.4835221
GTCTTAT950.001295625924.7613181
Produced by FastQC (version 0.11.2)