FastQCFastQC Report
Sun 14 Apr 2019
SRR6576233_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576233_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403106
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8990.22301826318635795No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6090.15107688796495214No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4620.11461005293892922No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4450.11039279990870887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.10146214643294817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA452.2035238E-762.650294
CCGAACT405.6761474E-446.9935461
GTATCAA10850.044.1782651
CGCTAAA555.2851123E-542.7161065
GTACATG25350.036.705021
TACATGG25200.036.3640562
ACATGGG25350.036.14443
TAGTACC651.4140338E-436.14444
AACGCAG13150.035.7321056
CCCGCTA801.1588596E-535.2407883
ATCAACG13850.033.926153
CAACGCA13950.033.6829575
TCAACGC13950.033.6829574
CATGGGT6600.031.3251464
CGCAGAG15300.030.7109268
ACGCAGA15200.030.6038447
ATAGACT1701.2187229E-1030.403824
CATGGGA12100.029.9012764
ATAGCAC1303.3301512E-728.9155223
ATGGGTA3900.028.915525
Produced by FastQC (version 0.11.2)