FastQCFastQC Report
Sun 14 Apr 2019
SRR6576232_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576232_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445701
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8400.18846715623254154No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5490.12317674853769679No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4970.11150973410425376No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4770.1070224208606218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11650.047.7551731
ATCAACG13450.039.8453753
TCAACGC13600.039.7515684
AACGCAG13650.039.6059576
CAACGCA13700.039.461415
GTACATG25900.039.1383971
TACATGG26000.038.69332
ACATGGG26250.037.9666143
TAACGCA500.00169903537.608444
CGTCTAC500.001700909237.60000294
ACGCAGA16050.033.383187
CGCAGAG16250.032.972318
CATGGGT6650.032.5185744
TATCAAC17000.031.8012542
TATAACG600.004153708531.3403642
AGTGCGT600.00415827431.3333348
CATGGGA13800.029.9777414
GGTACCT3400.029.0294118
CATGGGG9700.028.5940464
GCAGAGT19150.027.733689
Produced by FastQC (version 0.11.2)