FastQCFastQC Report
Sun 14 Apr 2019
SRR6576232_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576232_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445701
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9130.20484584957179813No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5490.12317674853769679No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG250.005235303756.3962944
GTATCAA10200.049.328351
CGCGATA205.6715874E-447.0074682-83
TACATGG26450.036.978622
GTACATG26750.036.915481
ATCAACG13650.036.1514743
TCAACGC13750.035.8885544
CAACGCA14350.035.37055
AACGCAG14500.035.3287166
ACATGGG26950.035.2258873
CATGGGA13100.031.5704444
TATCAAC16100.031.5435752
AGACCGC600.004159610731.3312746
CGCAGAG16650.030.766758
ACGCAGA16700.030.6746337
CATGGGT6450.028.416744
GCAGAGT19050.026.8906219
CGTCATG350.00883780326.85538120-21
ATGGGTA3650.025.7517345
TATAAGG1501.1548618E-625.0790882
Produced by FastQC (version 0.11.2)