FastQCFastQC Report
Sun 14 Apr 2019
SRR6576231_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576231_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399250
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11920.2985597996242956No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6360.15929868503443959No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6150.15403882279273637No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6130.1535378835316218No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5450.13650594865372573No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5330.1335003130870382No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5170.1294927989981215No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4660.11671884783969944No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4410.11045710707576707No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4210.10544771446462117No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.10269254852849091No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4050.10144020037570446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11700.038.964461
TACATGG25600.037.8190042
ACATGGG25200.037.663863
GTATAGT1003.3185643E-837.5986981
GTACATG25600.037.4518281
CATGGGG10300.036.9506264
CATGGGT6200.035.6188744
ATGGGTA4150.033.9662175
AACGCAG13700.032.5819476
GTCTATG1052.0614607E-631.3322471
CAACGCA14200.031.1038075
GGGTACC3950.030.9278897
TCAACGC14550.030.3556044
ATCAACG14650.030.14843
GGTACCT3650.029.6079968
TGGGTAC4200.029.0869436
GATATAC650.006150463628.9220731
GTATTGG1005.3162075E-528.1990241
ATGGGGG3350.028.05175
ACGCAGA16100.027.7250127
Produced by FastQC (version 0.11.2)