FastQCFastQC Report
Sun 14 Apr 2019
SRR6576230_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576230_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444095
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12200.27471599545142367No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7170.16145194158907442No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6350.1429874238620115No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6120.13780835181661583No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5960.13420551908938402No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5590.12587396840766052No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5340.12024454227136086No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4530.10200520158974995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGT6800.036.6319124
GTACATG29400.035.1696471
GTATCAA12700.034.7869871
TACATGG29350.034.7491572
ACATGGG28650.034.1217583
ATGGGTA4550.032.0215955
GGGTACC4100.029.8045227
CATGGGG11100.028.3690284
GGTACCT4200.027.9758578
AACGCAG15800.027.6642276
ATGGGGA6100.026.9668885
TAACCTT1057.4120995E-526.8568215
ATCAACG16150.026.4826553
ATGGGAG5550.026.2519365
TCAACGC16300.026.2389494
CAACGCA16500.026.2057465
ACGCAGA18350.023.563757
ATGGGTG3000.023.4997185
GTACCTG5050.023.2670469
CGCAGAG18600.023.2470328
Produced by FastQC (version 0.11.2)