FastQCFastQC Report
Sun 14 Apr 2019
SRR6576229_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576229_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356883
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7640.21407576152408492No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.11320236604153182No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3830.10731808463838288No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3770.10563686138034033No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3680.10311502649327652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10050.043.1192471
GTACATG23800.039.1866071
TACATGG24200.038.4470982
ACATGGG24700.037.859063
ATCAACG11800.035.840513
AACGCAG12050.035.0969356
TCAACGC12150.034.808074
CAACGCA12450.034.3467565
AACGAAC702.1737136E-433.59316694
ATGGGTA3650.033.4729655
CATGGGG8900.032.735234
GGGTACC3100.031.832547
CATGGGA11600.031.5973264
CATGGGT6250.030.8260274
TATCAAC14400.030.0219572
CGCAGAG14300.029.5954198
GTACTAG804.71348E-429.439431
ACGCAGA14500.029.1667617
ATGGGGA5000.028.1945385
TGGGTAC3750.027.567996
Produced by FastQC (version 0.11.2)