FastQCFastQC Report
Sun 14 Apr 2019
SRR6576224_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576224_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183338
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5400.2945379572156345No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2980.16254131713010941No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2890.1576323511765155No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2760.15054162257687986No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2480.1352692840545877No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.1352692840545877No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2410.13145119942401465No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2360.12872399611646249No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2330.1270876741319312No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2040.1112698949481286No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1990.10854269164057642No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1960.10690636965604511No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1950.10636092899453468No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1930.10527004767151382No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT1870.10199740370245121No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATA450.001012461441.7715952
ATGGGAG1401.2732926E-1136.9231035
CATGGGG5850.034.5418974
TGGATAG550.0027130534.176765
GATAGTG550.0027130534.176767
ATGGGGA2550.033.1715625
GTACATG14500.032.7598951
TACATGG14600.032.1870162
ACATGGG14800.031.7520583
GTATATA600.00413833231.3543551
AATACTG753.2677295E-431.3286975
TGGGAGT1252.3479333E-730.0755486
CATGGGT2850.029.6798174
GTATCAA8500.029.3255441
GAGTGGT650.006120767428.942489
ATAAGGT650.00614530928.9187974
TGGGTAC2002.5465852E-1128.1958276
GTATAAT856.767492E-427.6656071
TATAATG856.7999784E-427.642972
ATGGGTA2400.027.412615
Produced by FastQC (version 0.11.2)