FastQCFastQC Report
Sun 14 Apr 2019
SRR6576224_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576224_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183338
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6400.34908202336667793No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3210.1750864523448494No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3160.17235924903729724No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2810.15326882588443203No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2650.14454177530026507No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2620.1429054533157338No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2460.13417840273156684No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2270.12381503016286857No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2240.12217870817833729No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2150.11726974222474337No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2140.11672430156323294No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2030.11072445428661816No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG1980.10799725097906598No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC1960.10690636965604511No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.10581548833302425No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1920.10472460701000338No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1900.10363372568698251No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1880.10254284436396165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA2200.044.850625
CATGGGT2950.044.5972254
TGGGTAC2300.038.814826
TCGATAA250.001702941537.5890966-67
CTCGATA250.001702941537.5890964-65
CGATAAT250.001702941537.5890966-67
TAGTACT1003.3092874E-837.589094
GGTACCT2300.036.7719358
TACATGG13050.036.3748442
CCTAGTA651.4090037E-436.1532172
AGTACTA651.4112682E-436.1433565
ACATGGG13050.036.0048753
GGGTACC2350.035.989557
GTACATG13350.035.909491
CATGGGG5800.035.6448254
ATGGGAG2150.034.9665955
GTATCAA7100.034.4219361
GTACTGT1107.6557626E-834.17196
GTATAAT702.1780934E-433.5708471
ATGGGGA2200.032.0361565
Produced by FastQC (version 0.11.2)