FastQCFastQC Report
Sun 14 Apr 2019
SRR6576222_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576222_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161609
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3890.24070441621444352No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2100.12994325811062502No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1960.12128037423658336No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.11756770971913692No Hit
GTACATGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG1740.10766727100594645No Hit
ACTTTATTCTGTAGACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCT1710.10581093874722325No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1680.10395460648850002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTAT1101.7753337E-938.4564441
ACATGGG12750.038.327293
GTACATG12700.038.1199951
TACATGG12700.038.1199952
GTATCAA6000.037.601861
CATGGGG6000.036.0239644
TTTATTC1309.10768E-932.5300033
AACGCAG7100.032.4281856
GTACTTG902.579386E-531.334881
ATGGGAT1201.641165E-731.3251885
TCTGTAG1201.641165E-731.3251888
GTACTGT600.004155791431.3251886
CGCCATA300.00416189231.32518866-67
TATTCTG1351.315675E-831.3251865
CAGTACT902.5848509E-531.3251864
ATCAACG7300.030.8960743
TCAACGC7300.030.8960744
ATGGGGA3200.030.8357285
ATGGGAG2150.030.5966935
CAACGCA7600.030.2947525
Produced by FastQC (version 0.11.2)