FastQCFastQC Report
Sun 14 Apr 2019
SRR6576217_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576217_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764236
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17780.232650647182284No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11810.15453341637923365No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA10530.13778466337623457No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10320.13503682108668003No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9470.12391460229562595No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8430.11030624048068921No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG7950.10402545810456457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19150.044.4305571
AACGCAG24550.034.2679566
ACGTATA550.002716783734.180947
TCAACGC24750.033.9910474
CAACGCA24950.033.53025
ATCAACG25550.032.9267463
GTACATG41400.032.2470471
ACATGGG40250.032.227743
TACATGG42350.031.3016822
ACGCAGA28800.029.2110547
GTACGTA1005.3221644E-528.1992746
CATGGGG21450.028.0458974
CGCAGAG30300.027.609858
CATGGGT9800.026.8564534
TACGTAG1406.3650623E-726.8564517
TATCAAC32450.026.5099122
ATGGGTA7000.026.1850385
GGTATCA10100.025.5984271
TAATACC1351.4569016E-524.3697434
GCAGAGT34700.024.1088899
Produced by FastQC (version 0.11.2)