FastQCFastQC Report
Sun 14 Apr 2019
SRR6576214_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576214_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292403
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.16142105245158223No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4370.14945127101979117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6200.050.9486731
TCAACGC8000.039.3569154
CAACGCA8150.038.6325535
ATCAACG8150.038.0494423
TAGGCAT1055.0962626E-835.804445
AACGCAG8850.035.5768746
TATCAAC9150.034.4104162
GACTTAT550.002715600234.1769687
ACGCAGA10050.031.3288847
CTTACCC902.5929041E-531.323533
CGCAGAG10300.030.5684788
GTACATG12500.028.2879221
TACATGG12250.028.0041892
ATTACAC856.8163115E-427.6384093
GCAGAGT11400.027.6188879
ACATGGG12500.027.4394133
CGTATGG350.0088312826.85792520-21
CCCCATA700.00883155426.853332
CCTTATA909.508524E-426.1074072
CCTTACC909.508524E-426.1074072
Produced by FastQC (version 0.11.2)