FastQCFastQC Report
Sun 14 Apr 2019
SRR6576214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292403
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4090.13987544587435835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6100.047.7916151
CCGAACT608.785022E-539.1839871
CAACGCA7350.037.7252165
ATCAACG7500.036.970713
TCAACGC7600.036.4842534
TATCAAC8400.034.134372
AACGCAG8200.033.8146746
TACATGG12950.030.8525072
GTACATG13200.030.6347541
GTCTTAA1102.9482944E-629.922321
ACATGGG13550.029.1344643
CGCAGAG9550.029.0345888
AGACGTC650.00614890128.9210246
ACGCAGA9650.028.7337137
GTACCGG700.00880642926.8690221
TGCCCGC1057.380733E-526.869021
TAGGACA909.504593E-426.1092594
TATACCT909.504593E-426.1092594
GCAGAGT10950.025.3224059
GGGTACC1901.3253157E-824.7350867
Produced by FastQC (version 0.11.2)