FastQCFastQC Report
Sun 14 Apr 2019
SRR6576212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293526
Sequences flagged as poor quality0
Sequence length100
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8370.285153615011958No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5300.18056322097531394No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4550.15501182178069406No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3840.13082316387645387No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3400.11583300968227687No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3380.115151639037087No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3360.11447026839189714No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3340.1137888977467073No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3340.1137888977467073No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3250.1107227298433529No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3170.10799724726259342No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA3160.10765656193999851No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC3120.10629382064961879No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3110.10595313532702384No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3070.10459039403664411No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3010.10254628210107453No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3000.1022055967784796No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2980.10152422613328974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6450.045.1919751
ATTATGC802.450961E-741.1234363
GTATACG651.4084161E-436.164791
GTACATG15650.034.2467881
TATTTAG550.002709702434.1921652
GTACTAG550.002709702434.1921651
AACGCAG8650.033.686586
ATCAACG8800.033.1123773
TACATGG16350.033.0681152
TCAACGC8850.032.92534
CAACGCA9000.031.8543435
GGTACCT3550.031.7734388
ACATGGG17350.030.880673
AAGGGTA1252.353845E-730.0788545
CATGGGG8300.030.0109064
TAGGACA804.773376E-429.3738824
GGGTACC3850.029.2975867
GATTTAT650.006137719428.9318331
TATACGG650.006137719428.9318332
TGGGTAC3900.028.9219786
Produced by FastQC (version 0.11.2)