FastQCFastQC Report
Sun 14 Apr 2019
SRR6576211_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576211_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450598
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11950.2652031300627166No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8080.179317262837385No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7540.16733318834082708No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7430.16489198798041713No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6310.14003612976533408No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5650.1253889276028744No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5550.12316965454795628No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5510.12228194532598902No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5260.11673376268869369No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.11296099849533286No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5030.11162943466238198No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4880.1083005250800048No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4700.10430583358115215No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4650.10319619705369308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCTT200.002163764670.486526
CGAATAC301.3715577E-462.654683
GTATCAA10750.047.2148131
ATCAACG12850.038.0316353
GTACATG23950.037.283021
ACATGGG23700.037.2755663
TCAACGC13250.036.883514
TACATGG24100.036.6609572
AACGCAG13800.036.435066
CAACGCA13750.036.2257965
ATGGGTA4850.034.8799255
GGACCGT550.002717810734.175286
CATGGGT7400.032.3856964
GGTACCT4250.032.0608988
GGGTACC4450.031.6758167
ACGCAGA16350.030.7491137
CGCAGAG16850.029.8366788
TATCAAC17300.028.5237222
TGGGTAC5300.028.3719316
TGCCCGC1205.8364876E-627.4144651
Produced by FastQC (version 0.11.2)