FastQCFastQC Report
Sun 14 Apr 2019
SRR6576210_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576210_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493528
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12340.250036472094795No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8180.1657454085685108No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7410.1501434569061938No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7380.14953558865961No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6060.12278938580992366No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6020.1219788948144786No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5810.11772381708839215No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5660.11468447585547324No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5610.11367136211116693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.10840317064077418No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5290.10718743414760662No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4980.10090612893290755No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4950.10029826068632378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10300.050.6739731
GTACATG26650.040.7581061
TACATGG27150.039.83432
ACATGGG26800.039.6366353
CATGGGT7750.039.4216464
ATCAACG14350.035.374883
TCAACGC14600.035.0910844
CAACGCA14600.034.4472125
AACGCAG15450.032.2445646
CATGGGG8200.031.5262244
ATGGGTA5400.031.3351575
GTATACG804.7640246E-429.3886221
GGGTACC4500.029.2431857
CATGGGA14050.029.1048974
ACGCAGA17250.028.6074627
GGTACCT4650.028.2998548
CGCAGAG17750.027.8016178
TATCAAC18900.026.8695952
TGGGTAC5050.026.0582836
GTATAGT2251.1641532E-1025.078291
Produced by FastQC (version 0.11.2)