FastQCFastQC Report
Sun 14 Apr 2019
SRR6576209_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576209_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275282
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4640.16855442782310504No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.11043221133237915No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2860.1038934619771725No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAA250.005237748456.383875
GTATCAA7300.041.2236481
CAGTACG651.4130748E-436.143519
ATCAACG9100.032.012823
GGTATCA4050.031.3471491
TACATGG15450.031.0428092
TCAACGC9600.030.3454864
GTACATG16200.030.1861441
CAACGCA9750.029.8786325
AACGCAG9750.029.8786326
ATTAGTC650.00615474628.9148063
TCATACC650.00615474628.9148064
ACATGGG16950.028.5523013
GTCCTAC856.7853136E-427.6592481
TATCAAC11100.027.5346582
TGCCCGC1205.802107E-627.4287571
TAATACC700.00883687926.8494634
ATACCAT1101.0175588E-425.629036
TATAAGG1301.0802116E-525.318852
CATGGGT4650.025.2615914
Produced by FastQC (version 0.11.2)