FastQCFastQC Report
Sun 14 Apr 2019
SRR6576207_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576207_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207432
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3080.14848239423039838No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.13450190905935439No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2210.10654093871726637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6950.043.288461
GTGTAAG757.404893E-637.6068531
CAAACGC500.00169729237.606852
ACATGGG13200.036.663243
AACGCAG8350.036.013146
ATCAACG8550.035.170733
TACATGG14150.034.882682
CAACGCA8700.034.564345
CATGGGT3400.034.5484544
TCAACGC8750.034.366834
GACGTGG550.002715894334.1715628
GTACATG14750.033.7824251
GCATAGG600.0041494931.339041
ATGGGAT1950.031.3239295
TATCAAC9500.031.17412
CGCAGAG9750.030.8420268
ACGCAGA9850.030.528917
CATGGGA6650.029.6753044
GTCCTAA650.006137234628.9283451
ATAATAC650.00615171828.9143983
Produced by FastQC (version 0.11.2)