FastQCFastQC Report
Sun 14 Apr 2019
SRR6576205_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576205_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211308
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5550.26264978136180367No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.17036742574819694No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3200.15143771177617504No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3090.14623204043386903No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3050.14433906903666685No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2860.13534745489995648No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2850.13487421205065592No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2830.13392772635205483No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2650.12540935506464498No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2440.11547125522933348No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2430.11499801238003295No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2360.11168531243492912No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2180.10316694114751927No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2130.10080072690101653No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATA352.933839E-453.7075272
GTATCAA6150.049.7391621
TAGACGA500.001695986837.6130689
ATCAACG8650.034.770193
GAGTGAT550.002713690334.1775177
CTTATGT550.002713690334.1775173
CAACGCA8850.033.984425
TCAACGC8900.033.79354
AACGCAG8800.033.109476
GTACATG12950.032.3430141
TACATGG13250.031.9205112
ACATGGG13100.031.5685463
CTTGTAC753.268983E-431.329393
ATGGGAG2750.030.7597645
AGTTTGC804.7581422E-429.3852088
GGTATCA2750.029.0921941
CCAGCTA650.00612544228.93998194
ACGCAGA10150.028.7057467
TAATACT856.8025646E-427.643584
TATCAAC11100.027.5190582
Produced by FastQC (version 0.11.2)