FastQCFastQC Report
Sun 14 Apr 2019
SRR6576204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229584
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5780.251759704509025No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3750.16333890863474806No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3440.14983622552094222No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3080.1341556902920064No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2850.12413757056240853No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2780.12108857760122656No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.11978186633214859No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2690.11716844379399262No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2530.11019931702557668No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2500.10889260575649871No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2460.10715032406439474No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2330.1014879085650568No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG255.5340453E-575.2434161
GTATCAA6650.039.6017951
CCGACCA608.805991E-539.1636739
GTACATG13500.037.6217081
ACATGGG14250.034.9587333
TACATGG14550.034.906742
GTGCCGC550.002713519634.1792036
CATGGGT3200.033.7786674
TAGGCGG702.1805604E-433.568865
GGTACCT2300.032.6931538
ATGGGTA2350.031.9975555
TAATCAG600.004155666631.3309385
TAGTTCC753.2689096E-431.33093894
TAGTATT600.004155666631.3309387
CATGGGG6500.031.0899314
TGGGTAC2300.030.6498326
ATCAACG8850.030.2688733
ATGGGAG2550.029.4879425
TCAACGC9100.029.437314
CGGTCGG404.778602E-429.37275360-61
Produced by FastQC (version 0.11.2)