FastQCFastQC Report
Sun 14 Apr 2019
SRR6576202_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576202_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417138
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8980.21527647924667617No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5090.1220219687489512No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4830.1157890194611855No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4620.11075471426722093No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4260.10212447679185306No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG205.6839595E-446.98633622-23
ACATGGG23800.032.9733543
TACATGG24400.032.9407962
GTACATG24600.032.860111
ATGGGTA4100.032.0920755
GTATCAA12200.031.9737821
CAGTCCG600.00416389431.3242239
CATGGGG10750.030.1622854
CATGGGT6250.030.0748624
TGGGTAC4250.028.7480286
GGTACCT3950.027.3624118
GTATAAC1057.413006E-526.8557721
AACGCAG15150.025.4345956
TCAACGC15300.024.8781014
GGGTACC4350.024.8463277
CCTAACT950.001302669424.7385442
CAACGCA15550.024.4781325
ATCAACG15550.024.4781323
TATAGAG1401.9300447E-523.4959835
TCTATTC1401.9300447E-523.4959833
Produced by FastQC (version 0.11.2)