FastQCFastQC Report
Sun 14 Apr 2019
SRR6576201_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576201_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460674
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9140.19840494579681078No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6260.13588785127877848No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5340.11591711275218484No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5180.11244394083451638No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4690.10180735183665673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12400.046.7302931
TCAACGC15850.034.696094
AACGCAG15750.034.6141936
ATCAACG16000.034.0770453
GTACATG25450.033.874931
CAACGCA16100.033.8617135
TACATGG25550.033.665432
ACATGGG25900.032.4845773
TAACCGT300.00416154531.33181232-33
CATGGGG11300.029.9487024
ACGCAGA18150.029.5192287
CGCAGAG18200.029.4381318
TATCAAC18850.029.1741662
GCACGTT650.0061518528.9216756
GGGTACC3850.028.0765617
GGTATCA6000.027.480961
GGTACCT3700.026.6743838
GCAGAGT20900.025.859999
ATGGGTA4550.025.8229245
GTATAAC1301.06642765E-525.3670391
Produced by FastQC (version 0.11.2)