FastQCFastQC Report
Sun 14 Apr 2019
SRR6576200_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576200_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293714
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9310.31697501651266197No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6240.2124515685326542No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6200.21108969950359874No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5400.1838523189224892No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4850.16512661977297644No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4630.15763634011317132No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4480.15252933125421328No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4360.14844372416704685No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4070.13857017370639466No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4050.13788923919186694No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3960.13482503387649208No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3930.13380363210470048No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3780.12869662324574244No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3750.12767522147395086No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3680.12529195067310378No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3640.1239300816440483No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3490.11882307278509026No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG3380.11507793295518771No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.11507793295518771No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG3380.11507793295518771No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3370.11473746569792383No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3330.11337559666886834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11100.040.2676121
CTTAGGT1209.640644E-1139.1545373
GGGTATA500.001691190937.6396181
CGCGAAG250.001702515637.59475726-27
CAACGCA12250.034.9094165
GGGTACC2700.034.809967
TCAACGC12300.034.761594
GGGTTAG550.002699750234.217831
GTATATC550.002717856334.171233
AACGCAG12600.033.5667466
ATCAACG12750.033.534713
GTACATG18800.033.034771
ATGGGTA4000.032.8954125
AGGTATA1001.4079615E-632.8954126
TAGGTAT1151.1338125E-732.6910935
ACATGGG18600.032.334073
GCCTTAG902.5688418E-531.3663461
GGTACCT3000.031.3503138
TACATGG19900.031.1662272
ACGCAGA14150.029.8898227
Produced by FastQC (version 0.11.2)