FastQCFastQC Report
Sun 14 Apr 2019
SRR6576196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248048
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5150.20762110559246597No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2840.11449396890924338No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2770.11167193446429723No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2740.1104624911307489No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2710.10925304779720055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT1950.043.3696335
CTATAAT757.4376494E-637.5870131
CAAACGC550.002717518534.1700172
GCAAACG550.002717518534.1700171
TACATGG16300.033.724552
GTACATG16950.032.9856571
GTATCAA8700.032.9426461
ACATGGG16800.031.8818453
CATGGGT3000.031.3225124
AAACGCA600.004161779331.3225123
GACGCGA300.004165758431.32251262-63
CATGGGG6450.029.8656544
GTGTAAC650.0061553728.913091
AACGCAG10500.027.7427966
GGGATTG856.814663E-427.6375127
GACAATA700.00883778226.8478687
TATATCT700.00883778226.8478684
GTTATAG700.00883778226.8478681
ATCAACG10800.026.5371283
GGTACCT1956.020855E-1026.5036648
Produced by FastQC (version 0.11.2)