FastQCFastQC Report
Sun 14 Apr 2019
SRR6576195_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576195_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271633
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4420.16271955174813074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3500.12885032378245648No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3120.11486086005750407No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACC250.00523288456.3969088
GTATCAA8550.032.0168571
ATGGGTA2100.031.3373835
TACATGG18600.030.8319422
GTACATG18600.030.4498461
ACATGGG19050.029.6101263
CATGGGT3400.029.0331634
ATCAACG9600.028.8891493
CAACGCA9550.028.5481935
TCAACGC9750.028.4447024
AACGCAG9450.028.352876
GGTACCT2002.5465852E-1128.1984548
GGGTACC2203.6379788E-1227.7712027
CATGGGG8000.027.0284924
GTAGGAC700.00881879726.8606153
TACACTG1755.506081E-926.8606155
AGGCTAT1057.39457E-526.8606156
CCGAGTC700.00882672126.855679
GTATTAT909.2694064E-426.2207011
TACTACA950.00130104224.7400382
Produced by FastQC (version 0.11.2)