FastQCFastQC Report
Sun 14 Apr 2019
SRR6576195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271633
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5140.18922590406909323No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3170.11670157896868201No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.1056572655016143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7050.036.6654471
TACATGG15200.033.0844272
GTACATG15800.032.125511
CGATTGT300.00415974531.3322968-69
CGCCGGT902.5872825E-531.3322897
ACATGGG15750.030.7354833
ATATGGT1154.181984E-628.6077424
AACGCAG8650.028.2533956
GTCTTAA1354.6141213E-727.8509251
GTATAGA856.803921E-427.646141
TCAACGC9050.027.5239474
CAACGCA9150.027.2231375
ATCAACG9150.027.2231373
CATGGGC2156.548362E-1126.2316864
TATCAAC10250.025.2186722
CCTACAC950.001302275324.736023
TATATCT950.001302275324.736024
ATGGGAG3900.024.1017635
CATGGGA10200.023.9599884
ACGCAGA10600.023.4992187
Produced by FastQC (version 0.11.2)