FastQCFastQC Report
Sun 14 Apr 2019
SRR6576194_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576194_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318022
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10040.3157014294608549No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5770.18143398884353915No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5110.16068070762400086No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5050.15879404569495192No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4950.15564960914653703No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4660.14653074315613385No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4240.13332410965279132No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3820.12011747614944879No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3810.1198030324946073No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3710.1166585959461924No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3520.11068416650420411No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3450.10848306092031369No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.1081686172654722No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3410.10722528630094774No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3290.10345196244284988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCCG450.001013574141.7716984
GTACATG19650.040.2342951
TACATGG19750.039.735992
GTATCAA8300.039.1219641
GGTACCT3500.038.9678238
ACATGGG19750.038.784233
ATGGGTA3750.037.594535
TATTAGT1003.3182005E-837.594534
GGGTACC4000.035.244877
CATGGGG9400.034.9949074
TGGGTAC3900.034.943636
ATGATAG550.002716005334.176843
CATGGGT5400.033.9395034
ATGGGGG3900.033.7386785
ACCTAAC600.004130724431.3731691
ATTAGTG1201.6480226E-731.3287735
TTAGTGC1201.6480226E-731.3287736
TGGGTTA804.7772683E-429.3707266
TCAACGC11050.028.9188694
ATCAACG11100.028.78863
Produced by FastQC (version 0.11.2)