FastQCFastQC Report
Sun 14 Apr 2019
SRR6576194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318022
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10170.31978919697379427No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6180.1943261786920402No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5560.17483067209186784No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5530.1738873411273434No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5060.1591084893497934No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4780.1503040670142317No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4310.13552521523668173No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3970.12483413097207112No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3890.1223185817333392No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3710.1166585959461924No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3630.11414304670746048No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3580.11257082843325304No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3570.11225638477841154No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3540.11131305381388709No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3470.10911194822999666No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3390.10659639899126476No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3320.10439529340737434No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3220.10125085685895946No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3200.10062196954927645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTCG555.2822077E-542.7166255
AATAAGG450.001014091441.7673657
GTATCAA8150.038.6344831
ATATGAT500.001702259837.590638
TACATGG18500.034.8022042
GTACATG18550.034.2017061
TATTAGT550.002717386234.1732984
ACATGGG19150.033.12493
CATGGGG10200.031.7861944
GTATACA600.004158370731.3304481
CATATGT600.00416157331.3255233
AACGCAG10200.029.9435166
GTATATC804.7757692E-429.37231
TTAGTGC804.7801697E-429.367686
TCAACGC10950.028.3217074
CAACGCA11100.027.9389825
ATACATA856.808835E-427.6445141
TAGTGCT856.8150985E-427.640177
CTTATTA700.008830545526.8546711
GTATATG700.008830545526.8546711
Produced by FastQC (version 0.11.2)