FastQCFastQC Report
Sun 14 Apr 2019
SRR6576192_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576192_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301939
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5780.1914293946790577No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3290.10896240631385809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6750.049.6075671
CGGGCGT555.2750704E-542.725546
CAACGCA8250.040.4468425
ATCAACG8350.039.9624523
TCAACGC8450.039.489524
TACGGGC608.81073E-539.165084
AACGCAG8750.038.1355976
GGCGTCG651.4113593E-436.152388
ATACGGG651.4113593E-436.152383
TATCAAC9750.034.235592
GACCGCG550.002714390434.180437
TATACGG702.1774799E-433.5811922
CGTACGA352.1842985E-433.5700760-61
GCGTACG352.1842985E-433.5700758-59
GTACATG13400.033.0838781
TACATGG13500.032.7354432
ACGCAGA10250.032.554787
CGCAGAG10300.032.3967488
ACATGGG13500.031.3320643
ACTGCGT902.5883603E-531.3320628
Produced by FastQC (version 0.11.2)