FastQCFastQC Report
Sun 14 Apr 2019
SRR6576192_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576192_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301939
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4970.16460278400604095No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3720.12320369346126206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA353.63226E-667.1422653
GTATCAA6350.055.557331
CCGCTAA503.0042724E-546.9995844
CGCTAAA555.2740834E-542.72695
GGTATCA2850.039.6113971
CAACGCA9100.038.2194445
ATCAACG9100.038.2194443
TCAACGC9200.037.8040164
AACGCAG9500.037.104946
TATCAAC10150.034.265712
CGCAGAG10500.033.123528
ACGCAGA11000.031.6179057
GGTACCT2200.029.9088298
CATGGGT3150.029.8410074
ATGGGTA2051.8189894E-1229.8046175
GGGTACC2250.029.2441887
GTACATG11050.028.9467951
TACATGG11050.028.4974882
TGCCCGC1354.582671E-727.874691
GTACTAT700.00879067626.8791661
Produced by FastQC (version 0.11.2)