FastQCFastQC Report
Sun 14 Apr 2019
SRR6576190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260802
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3330.12768306991510803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6100.046.228851
GGCGTCG555.2722688E-542.726668
GTACATG12400.040.5558741
TACATGG12450.039.2604832
CAACGCA7150.038.1253285
ATCAACG7150.038.1253283
TCAACGC7150.038.1253284
ACATGGG12700.038.1175653
AACGCAG7350.036.448466
ACGACGG550.002713366234.181336
CATGGGT2550.033.1764
TATCAAC8450.032.2598952
CATGGGG5700.032.1574334
CGACGGT600.0041554331.3328887
CGCAGAG8700.030.7926658
GGGTACC1551.5079422E-930.322157
ACGCAGA8850.030.2707547
GTCTATG953.74165E-529.6837841
GTATACG650.00614599528.9226631
TATACTA650.00614599528.9226635
Produced by FastQC (version 0.11.2)