FastQCFastQC Report
Sun 14 Apr 2019
SRR6576187_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576187_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249345
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5350.21456215284044194No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3740.14999298161182298No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3520.14116986504642162No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3390.13595620525777538No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3280.1315446469750747No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.1251278349275101No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2840.11389841384427199No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2500.10026268824319719No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2500.10026268824319719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7350.040.3519331
TAAGGCC757.428567E-637.5938224
AACGCAG8850.031.8591736
GAAACCG600.00415813431.3281845
CAACGCA9350.031.1606545
TCAACGC9350.031.1606544
ATCAACG9350.030.6580643
TACATGG15850.030.5375692
GTACATG15350.030.362531
ACATGGG15450.030.1115553
GGTACCT2350.028.0122538
GACGCTA350.00883838926.85273248-49
TATCAAC11150.026.5516912
CATGGGT3950.026.1729154
ACGCAGA10600.026.156087
ATGGGTA2700.026.1068215
GGGTACC2409.094947E-1225.454157
CGCAGAG10900.025.4515028
TAAGAGT950.0013029724.7327774
CATGGGA6450.024.042564
Produced by FastQC (version 0.11.2)