FastQCFastQC Report
Sun 14 Apr 2019
SRR6576185_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576185_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428606
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10420.24311372215974578No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6770.15795392505004596No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6580.15352094931008897No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6530.1523543767469424No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5900.1376555624512956No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5680.13252264317345067No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5470.12762303840823508No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5360.12505657876931262No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5180.12085691754198495No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4980.11619062728939866No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4400.10265838555689841No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATCG500.001702174837.593985
GTATCAA12800.034.1748661
TACATGG29850.033.6897472
ACATGGG29200.033.313163
CATGGGG12150.033.2621654
GTACATG30150.033.2296751
TAAGGGT902.5929958E-531.3283164
AACGCAG15750.028.0463036
ATGGGGA7050.027.3289575
GCATATA1057.371977E-526.8779281
ATAATAC700.00883684826.8528443
TCAACGC16450.026.5671734
ATAGCAC1606.9056114E-826.4332683
CATGGGT7000.026.1815224
TAATACC909.5167995E-426.106934
CAACGCA16750.026.0913455
ATCAACG17000.025.707653
ATGGGTA3800.024.7328835
TATTGGG950.001304467924.7328832
ACGCAGA18500.023.8772587
Produced by FastQC (version 0.11.2)