FastQCFastQC Report
Sun 14 Apr 2019
SRR6576183_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576183_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201376
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3610.17926664547910376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.1430160495788972No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2260.1122278722389957No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2110.10477911965676148No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2020.10030986810742094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7100.043.8126221
GGTTAGC704.59844E-640.3082968
TACATGG14000.039.61682
TAATGCG308.823478E-539.15942818-19
GTACATG14400.038.6218571
ACATGGG14650.037.8496553
AACCGCG500.001700239337.593057
CCTATAG500.001700239337.593053
ATGGGAC1302.2919266E-1036.147165
CATGGGA9000.036.026674
CTATATA801.13197675E-535.3488161
ATGGGAG4200.034.6840635
GTATATA1107.456765E-834.277641
GTTCCGG550.002700974734.20947694
TCTACAC1001.4055458E-632.8939173
TCAACGC9250.032.5129054
ATCAACG9350.032.1651733
CAACGCA9450.031.8248025
AACGCAG9350.031.6625946
GGATAAG600.004096370731.421171
Produced by FastQC (version 0.11.2)