FastQCFastQC Report
Sun 14 Apr 2019
SRR6576183_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576183_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201376
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4330.21502065787382807No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.14698871762275545No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2450.12166295884315907No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2360.11719370729381853No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2180.10825520419513746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7500.036.36311
TATACTG651.4117871E-436.1432465
GTATAAG550.002705706534.1972731
TAGACCT550.002715615634.1717955
CCTATAC550.002715615634.1717953
TACATGG15150.033.8304672
GTACATG15550.033.5650061
ACATGGG15350.033.0587043
CATGGGG5700.031.3241464
GAATCTA600.00415887431.32414494
ATGGGAG3350.030.856625
CATGGGT2750.030.7546184
GTCTAGA650.006128725628.9361531
AACGCAG9300.028.2927766
TCCTATC1005.285736E-528.2127482
ATCAACG9300.027.7875483
TCAACGC9350.027.638954
TATAAGA1205.7914513E-627.4290642
CAACGCA9500.027.2025475
CTATAAG1057.3690084E-526.8692841
Produced by FastQC (version 0.11.2)