FastQCFastQC Report
Sun 14 Apr 2019
SRR6576182_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576182_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221869
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3340.15053928218903948No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.1334120584669332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6950.042.6804541
TACATGG14700.041.2441062
GTACATG15100.040.535891
ACATGGG15100.039.8402983
TAGTACT500.001699228837.5992774
AACGCAG8100.036.5548556
CAACGCA8200.036.1090665
ATCAACG8200.035.5359043
CATGGGA7150.035.4958274
TCAACGC8400.035.2493254
ATGGGAG3400.034.5581635
ACGCAGA9300.032.3434687
CATGGGG6250.032.335384
CGCAGAG9700.031.4942448
CTTACCT600.00415420431.3327333
CATGGGT3700.030.4859054
TATACTG804.769135E-429.3744355
GGTAAGG650.00609023628.9747641
GCAGAGT10400.028.9225239
TATCAAC10750.027.9808582
Produced by FastQC (version 0.11.2)