FastQCFastQC Report
Sun 14 Apr 2019
SRR6576181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635354
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10050.15817953455868697No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9680.1523560094057801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14650.041.414411
TACCGTC1353.45608E-1034.814327
ATACCGT1405.0931703E-1033.5709536
CATGGGT7800.031.9354424
ATCAACG18750.031.8342133
GTACTTG5050.031.665541
CAACGCA18750.031.583555
TCAACGC19000.031.4153424
AACGCAG19350.030.6042186
GGTATCA8200.030.3991471
CCGAACT804.7591157E-429.3954031
GTACATG32050.029.056051
ATGGGTA4900.028.7751035
TACATGG32200.028.4645672
ACATGGG32550.027.2899363
GTAATAG1755.502443E-926.8757971
TATCAAC22050.026.8588752
ACGCAGA24350.024.5129997
CGCAGAG24850.024.0197778
GCGTTAT1203.4193963E-623.5163211
Produced by FastQC (version 0.11.2)